| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.022737 |
| Chromosome: | chromosome 10 |
| Location: | 1927331 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGGCGGCAAGGGGCGGCGCAGCCCGCCGCGGAGCAAGGCCGCAGCG |
| Internal bar code: | ATGTCCACTTGGAGAACATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1965 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGGGGCGTCGACTAAGGA |
| Suggested primer 2: | TTCCCCTACTCCGAGGTCTG |