| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022749 |
| Chromosome: | chromosome 17 |
| Location: | 2129359 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g711850 | HTR5,HTR19,HTR30 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAAGTCCTGGGCAATCTCACGGACCAGGCGCTGGAACGGCAGCTTGCGG |
| Internal bar code: | GCTAATTCTGGAGTCCTAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5855 |
| LEAP-Seq percent confirming: | 90.2174 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGTGATGCCCTGGATGT |
| Suggested primer 2: | AGCTGCTCAATTCCTCCCAC |