Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.022756 |
Chromosome: | chromosome 8 |
Location: | 2497724 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g372500 | (1 of 1) K11373 - elongator complex protein 1 (ELP1, IKI3, IKBKAP) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACGTGGACGTGGTTTAGAAACAACAAAACGAAGCTCGCCCACGTGTGT |
Internal bar code: | GCTTGTGCCGAGGTCGGTGCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 759 |
LEAP-Seq percent confirming: | 72.4138 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAAATCCCGCCCACTAAGT |
Suggested primer 2: | AACCTCCCCAGACCCTATCC |