| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.022804 |
| Chromosome: | chromosome 10 |
| Location: | 2303126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g434300 | (1 of 1) K17868 - diphthamide biosynthesis protein 7 (DPH7, RRT2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGCGATGGTTGCGCGGCGCCGTCGGCTGCTCGCATCATTCACTCGG |
| Internal bar code: | GAGCCGCATTGAGGAAAGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2096 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAATCCCTCAGCTCACACT |
| Suggested primer 2: | GCGTTCACAGTGCAATGGTT |