| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022813 |
| Chromosome: | chromosome 7 |
| Location: | 3532894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACACGCAAGCAGAACGCTCGCTGACTTGTTGCCAGTAGCTGCCGTAGC |
| Internal bar code: | GTGTTTCTTTCAATCAGAAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3398 |
| LEAP-Seq percent confirming: | 72.4138 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGTGAAGTACGGCAGT |
| Suggested primer 2: | CGCATCCCACGTGTTTGTTT |