Insertion junction: CLIP2.022820_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.022820
Chromosome:chromosome 17
Location:5369775
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g738250 (1 of 1) 2.5.1.31 - Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl- diphosphate specific) / UPP synthetase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCGACCCATTTCCGCTACCGTGCCTCTCACCCAGCTACTGCCCCCGC
Internal bar code:TTGGGACGATGTCTAGTAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2211
LEAP-Seq percent confirming:34.1463
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCTGGGGGTAATTTGGGG
Suggested primer 2:ATATGGGTCCAAGCAACGCA