Insertion junction: CLIP2.022823_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.022823
Chromosome:chromosome 12
Location:2266495
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g508500 (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAAGTACGCACGCGCATGCACACACGCACGCACACACCCTGCAGGCGCT
Internal bar code:TGGCGGGGTTTGGTACACGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:100

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATTGCCGTGTACTTGCG
Suggested primer 2:CAAGCTCGACACTCCCTGTT