Insertion junction: CLIP2.022826_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.022826
Chromosome:chromosome 6
Location:5402348
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285550 PTK Protein tyrosine kinase; (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCGGCGCCCGCTGCCGCCGGCGCCAACGGCTGCGCACCCCCGCAACAT
Internal bar code:AATACAGTAAAGATTCCGAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2156
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCATGCAGCGTAGTCGAATG
Suggested primer 2:ACACCTCGCACACACAGTAG