Insertion junction: CLIP2.022830_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022830
Chromosome:chromosome 6
Location:6015998
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g290500 GEX63 (1 of 1) K08333 - phosphoinositide-3-kinase, regulatory subunit 4 (PIK3R4, VPS15) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATTCCTGTGCTGTCCAGCCGCTCACCAAGTGCCGCTTGTTTGCGCAT
Internal bar code:GCTATACTGGGCATGTCAATTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2160
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCGGTCTTCGTTACAACA
Suggested primer 2:TCCCTGCTCGTGACACTTTC