| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022837 |
| Chromosome: | chromosome 13 |
| Location: | 1193611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569850 | AMT4,AMT1D | Ammonium transporter; (1 of 8) K03320 - ammonium transporter, Amt family (amt, AMT, MEP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGGTGGCCTGGCCGGCCTGGCTGGCTGCATCATGGTGGGCCCCCGCA |
| Internal bar code: | GATTAGCTCTCAGCGTAGGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3175 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACAACGCTCCTACCTG |
| Suggested primer 2: | GGCTTCGCTTATGGCATTGG |