| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022843 |
| Chromosome: | chromosome 6 |
| Location: | 8863399 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311600 | RFK3 | Riboflavin kinase; (1 of 1) PTHR22749//PTHR22749:SF1 - RIBOFLAVIN KINASE/FMN ADENYLYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAGTAACACCCAGCTCGCGGCGCGCTTGCAACCCAACACAAAAATTA |
| Internal bar code: | GGAAAATTAGCCGCCAGATAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2544 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAACGCCTCACACTGAC |
| Suggested primer 2: | CGATGCCGTGTTGCTTATGG |