Insertion junction: CLIP2.022847_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.022847
Chromosome:chromosome 10
Location:6579558
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g465700 (1 of 1) PF01082//PF03712 - Copper type II ascorbate-dependent monooxygenase, N-terminal domain (Cu2_monooxygen) // Copper type II ascorbate-dependent monooxygenase, C-terminal domain (Cu2_monoox_C) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTTGCAATCAATCCCTACCCATAGGTTGGGGTGCCCCAGACCGTCATC
Internal bar code:TTCTTAATTTCCCGTATGGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:857
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAAGGGTAGGGACAGGGC
Suggested primer 2:AGGTGCGTGTGTGTATGTGT