| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022854 |
| Chromosome: | chromosome 6 |
| Location: | 4770915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279700 | (1 of 1) PTHR15535:SF22 - PROTEIN RLIB | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCGGCGGTGCGCGGTGACCCCAACGAGCTGCTCAATGCCGACCTGA |
| Internal bar code: | GGCTGCTTAATCATCTGCAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5704 |
| LEAP-Seq percent confirming: | 63.8889 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGTAGAAGCGCCACGA |
| Suggested primer 2: | ACAGACAACGTGACGCTGAT |