| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.022862 |
| Chromosome: | chromosome 8 |
| Location: | 1889955 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367550 | FAP291 | Flagellar Associated Protein 291 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCACTGAGGTCGCCGGCACCTTGCCGTCAGCCTCGCTATATGAATACA |
| Internal bar code: | GCAAAGATATGTATCCACTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1584 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTGACGCTGTTTTGGTC |
| Suggested primer 2: | CCAACCCACCTGCATGTTTG |