Insertion junction: CLIP2.022872_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.022872
Chromosome:chromosome 2
Location:3216144
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g096950 (1 of 1) PF09444 - MRC1-like domain (MRC1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCTCTCCGTCACCGTCGCCCTCCGCTGCGCTGCGCCGCCGCCGCCGCG
Internal bar code:AGCGTGCGCTTTATGCCAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:914
LEAP-Seq percent confirming:52.1739
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACCAGTACCCCGACCTC
Suggested primer 2:CCATGATCTTGCCAAGCACG