Insertion junction: CLIP2.022895_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022895
Chromosome:chromosome 4
Location:4050352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g232802 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGATGACACACCTACTGCTGTTGCCTGCGCAGGACTCGTTCCTCATCT
Internal bar code:AGAGGAAAGAATCATGCGGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2849
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCTGTGGCATCAATACG
Suggested primer 2:GACCCGGACTTCAAGGTCAG