| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022903 |
| Chromosome: | chromosome 16 |
| Location: | 5518508 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682250 | (1 of 16) PF07707 - BTB And C-terminal Kelch (BACK) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGACCGCCGTCAACAACGGACTTGAGTGCATGCTTTGTTTCAACCGAC |
| Internal bar code: | ATTTGATTGGTGGCTGGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2648 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCGGTCGACGGCTAAAT |
| Suggested primer 2: | GAAACAGTTGGAGGGCGAGA |