| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022904 |
| Chromosome: | chromosome 12 |
| Location: | 7660250 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACGGCCGCAGTTTCGGCCCCAGCGCGGGGCCCGGCTCCGTGAGCAGG |
| Internal bar code: | CCACATGGGTGAAGGTGACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1796 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCAGGTCAGCGCAAATCA |
| Suggested primer 2: | TGAGGTACGGTTTGTTCGGG |