| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022921 |
| Chromosome: | chromosome 12 |
| Location: | 4507838 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520850 | (1 of 1) K15153 - mediator of RNA polymerase II transcription subunit 31 (MED31, SOH1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAGACGTAAACCGACACGGAGCATGCCCACGTCCTCTTGTGCAGAGC |
| Internal bar code: | AAGGCCGGTATGAAAGTAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3748 |
| LEAP-Seq percent confirming: | 96.0526 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCAGCGCAGGACTTGGAA |
| Suggested primer 2: | GGGCTGTACACGGGTAAACA |