| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022928 |
| Chromosome: | chromosome 11 |
| Location: | 4311613 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATTGCTGACCGTGGGGATGTGGGATTGAGGGGTCGGCAGCTGGGGCA |
| Internal bar code: | TTCTTAGCCTGTGAGGTGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACACACACACACACAGC |
| Suggested primer 2: | GTTGATGGTCGCGATCAAGC |