| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.022968 |
| Chromosome: | chromosome 3 |
| Location: | 1332942 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGTCGTCGCTATCGCCGGCGTCGTCCCGCTCAGACTCAAAACCCTCA |
| Internal bar code: | TGGTTTTGGCACAGAGTTTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3700 |
| LEAP-Seq percent confirming: | 79.3103 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATACGCTCTTTCGCTG |
| Suggested primer 2: | ACCTATGTGGCTGTAGCTGC |