Insertion junction: CLIP2.023000_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023000
Chromosome:chromosome 10
Location:340525
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g420400 (1 of 1) K14289 - exportin-5 (XPO5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCAAGATCTTGCGCCCCCATGCAAGCCGCGACCTCCGACAGCCGACCAA
Internal bar code:CGGAAGATGTACATTACAACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2202
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCCCATACCGTATGCCT
Suggested primer 2:TCCGATCGCCAAGTCTATGC