| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023001 |
| Chromosome: | chromosome 6 |
| Location: | 7957715 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304400 | SOUL4 | SOUL heme-binding protein; (1 of 2) PTHR11220:SF35 - SOUL HEME-BINDING PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTACATGCGTGGTGATATGGATTAGGTACGGTATGCAACTGACCCTTC |
| Internal bar code: | CATAGAGTAGCACACGGCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 525 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGAAGGAGTGGGCATGG |
| Suggested primer 2: | GAACTGCGTGGTTTGTGGTC |