Insertion junction: CLIP2.023002_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023002
Chromosome:chromosome 1
Location:5217397
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g036451 SMM2 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCATTAGCCAATCAGCTGCACCCCGGTCACTCGCTACGCCCACCTCG
Internal bar code:TGGGGGGGTAGTCGGGAACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1427
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGGACGGACTGGACACC
Suggested primer 2:CGGTGGAGAAAGGTGAAGCT