Insertion junction: CLIP2.023016_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023016
Chromosome:chromosome 1
Location:6786390
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g048550 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGTTTAGGTCTAGGCCACGTGGGCACGGTCAAGCAGCATCCAGGGCGC
Internal bar code:CCGCGATGGTACTGTATGGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4843
LEAP-Seq percent confirming:91.1111
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:GTAATGACACCGCCAATCGC
Suggested primer 2:ATGATTGGAGCCCACGAGTG