Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.023017 |
Chromosome: | chromosome 10 |
Location: | 558175 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g421400 | (1 of 110) 3.4.19.12 - Ubiquitinyl hydrolase 1 / Ubiquitin thiolesterase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCATGGCTGGCGGGGCCGCTGGTGGCGCTCAACGTTGCCAACCTGTGC |
Internal bar code: | CCAGGTATGGCAAGGCTTCAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1958 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 21 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCGAACGTAAGGTTACCCC |
Suggested primer 2: | CCGCTTCACTTTGCTTTGCT |