| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.023044 |
| Chromosome: | chromosome 11 |
| Location: | 1594282 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g801266 | intron | ||
| Cre11.g801267 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGTGGGAAGCGCCGCCGGTTGTGTCCTCCACGCGCCATGCTCAAGCT |
| Internal bar code: | CCTGACAGTGCTCATTCTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3317 |
| LEAP-Seq percent confirming: | 1.02041 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCCTCAGCGAAACTTC |
| Suggested primer 2: | TCTGCAACCCAACCGACTAC |