Insertion junction: CLIP2.023046_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023046
Chromosome:chromosome 1
Location:6151376
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g043750 BBS7 (1 of 1) K16749 - Bardet-Biedl syndrome 7 protein (BBS7); Bardet-Biedl syndrome-7 associated protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACATCCCGCAAACCGGTAGAAAGAAGGCGATGAGCTCTTCGGTCTTGCGC
Internal bar code:CTGCACGCCTGGTGTGAGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:123
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCAGAACAACAAGCGCAA
Suggested primer 2:ACCGTACAGCCCTATCCTGT