| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.023052 |
| Chromosome: | chromosome 6 |
| Location: | 7660979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g302150 | RLS12 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; RegA/Rls-like protein 12 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGACTTTCGCGCCTGCGGCGTCCACTCCACCCGCGTCCAGCCCTGCAGC |
| Internal bar code: | TCTACAACAAAGGAAAGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1119 |
| LEAP-Seq percent confirming: | 27.907 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGACAGCTCCACCACCAG |
| Suggested primer 2: | ACACTAGTCCACCGCATGTG |