| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023053 |
| Chromosome: | chromosome 10 |
| Location: | 1912439 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCAAAAGCTATGCGGTCACCCCCCCCCCTTACCCACCTCGGATGCAA |
| Internal bar code: | TCTCGGTGCCGTCGTCCAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5181 |
| LEAP-Seq percent confirming: | 99.0291 |
| LEAP-Seq n confirming: | 102 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACGGAGATGCTACACG |
| Suggested primer 2: | TCTGGAGGGGGAACGTGTAT |