Insertion junction: CLIP2.023097_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023097
Chromosome:chromosome 12
Location:2812166
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g503600 Leucine-Rich Repeat Protein; (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGATGTGTCGCCGCCGCAACAACGGTGACGCACATAACGCCGGTGGC
Internal bar code:CCCGGTTCAACTCCATTAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1399
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCACGCTTTCTGTTTGC
Suggested primer 2:TCGGGAGAAGGATGGGTGAT