Insertion junction: CLIP2.023100_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023100
Chromosome:chromosome 12
Location:702061
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492850 POC19 Proteome of centriole protein 19; (1 of 1) PF10595 - Uncharacterised protein family UPF0564 (UPF0564) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCGTACGCCGCTGGCGGCGCCGGTCACCGGTGGGGGTCGACACGAG
Internal bar code:TAATTACAAGCTTGTAGCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:5880
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCATGTCGACCAACTCGT
Suggested primer 2:TCTCTGCTTGTTGACGGGTC