Insertion junction: CLIP2.023127_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023127
Chromosome:chromosome 16
Location:5096241
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g685600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACACACACACACACACACACACACACACACACACACAC
Internal bar code:CACGTAGCTCTGCTTACGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:347
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:AAACAGGCGGCACAGACATA
Suggested primer 2:CACTCGGTCTCTGTTTGCCT