Insertion junction: CLIP2.023137_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023137
Chromosome:chromosome 11
Location:1094357
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467673 PDE24 (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGGTACGTCACGTGCTGCTGGCGTGCGTGCGTGCGTGCGTGCGCATG
Internal bar code:AATGGCCCTGCTGTTGTGTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2120
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCATCGGGTCACTTTCA
Suggested primer 2:GTTGGTTGGGGGAATACGGT