| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023142 |
| Chromosome: | chromosome 2 |
| Location: | 3996671 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102750 | (1 of 2) PTHR31646:SF1 - ALPHA-1,2-MANNOSYLTRANSFERASE MNN2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGGATGGCAGCGATACGCAGCAGTGTCGACCCTGCCCCTTGTGCTTT |
| Internal bar code: | AGCTCACTAACGATTTTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1363 |
| LEAP-Seq percent confirming: | 85.9649 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTGCGTACGGTATGCTG |
| Suggested primer 2: | CCGCACACACCAGTAGAAGT |