Insertion junction: CLIP2.023145_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.023145
Chromosome:chromosome 8
Location:2227671
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g370250 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGTCCACCGCGGCCACCTGGTACAGCGGGCCGCCGCCGCCGGCGCCAC
Internal bar code:GGCCTGGCTCTCATCAGGAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1936
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCGGGCTGCAGGTACATT
Suggested primer 2:TGTGTTGAGAGGAGCGTGAC