Insertion junction: CLIP2.023165_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023165
Chromosome:chromosome 10
Location:1736832
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430200 ARS3 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGGCCCGGGTTTCAGCAAGAACTGCGGCACCGTACAGAAGTACGGCG
Internal bar code:TGTCGACGTGTAAGTCAACGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:5424
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGGCTAGCAGGGATAGC
Suggested primer 2:TGTTTCCGGTCGTCTCCTTG