| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.023177 |
| Chromosome: | chromosome 13 |
| Location: | 2841527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583050 | Tic55-like5,TIC55-5,PAO8 | Pheophorbide a oxygenase-related protein; (1 of 3) K13071 - pheophorbide a oxygenase (PAO, ACD1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGGGTCGTGTGGCGCTGGGAGCCTCCACCTGTTGCACAGCGTCACTTG |
| Internal bar code: | GTACAAAGGAGTTTAGGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGTTGTCCAGCGGCATTA |
| Suggested primer 2: | AACGAGGAGCACATTCACGT |