| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023182 |
| Chromosome: | chromosome 6 |
| Location: | 825663 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255450 | MAT3,RB | (1 of 1) K04681 - retinoblastoma-like protein 1 (RBL1); Retinoblastoma protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCTCATCGCGGGCACAAACACCGCGTTGTAGAAGCCGATGATGTCGG |
| Internal bar code: | GGTGGTCAGTTCAGAGATACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4691 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTTGAAGTGGTGGCGTC |
| Suggested primer 2: | TTCCTCTTCCTCCTCCTCGG |