| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.023226 |
| Chromosome: | chromosome 13 |
| Location: | 2246830 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578451 | TSL1 | (1 of 2) 6.1.1.4 - Leucine--tRNA ligase / Leucyl-tRNA synthetase; Leucyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACTGCGCTGCAAGCCACGTGCCGTCACCCCGCTCCTGCACCCACCC |
| Internal bar code: | ATTGTTAATGGTGCTCCAAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TGTTGACCCAGTCCGTGATG |