| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.023291 |
| Chromosome: | chromosome 12 |
| Location: | 4955470 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524600 | (1 of 1) PTHR17630//PTHR17630:SF50 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATTCAAGTGCGAGCCGCTCTACCTCCCAGCCTGCAACCGCCCTACCT |
| Internal bar code: | CCTTCCATATGGGGGTGTGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1719 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGAAAGCAAAACGGCGTG |
| Suggested primer 2: | ACTCCTTGGGGTGCTTTGAG |