| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023322 |
| Chromosome: | chromosome 14 |
| Location: | 1857748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619850 | ELG36 | (1 of 2) 2.7.7.52 - RNA uridylyltransferase / TUT; Exostosin-like membrane protein, a heparan sulfate glycosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGTTCGGTGCACGGCGTACCATGGCGCACCACCACCATGTACAAACG |
| Internal bar code: | CCAAACGGTTTAGAACATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2243 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCAAAGCTCGTGACTCCAG |
| Suggested primer 2: | ATATGATCGGAGCGGCATCG |