Insertion junction: CLIP2.023325_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023325
Chromosome:chromosome 17
Location:2776878
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g718000 PHC7,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCTTTGTGCTTTCTCTCGCACACACACACACACACACACACACACAC
Internal bar code:TCGCTTTCTATTAGGCGTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:5045
LEAP-Seq percent confirming:90.4348
LEAP-Seq n confirming:104
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:115

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTCGTACAGGACCAAGCA
Suggested primer 2:CGTGTTGTCCGTGACCAGTA