| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023332 |
| Chromosome: | chromosome 9 |
| Location: | 5101720 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g406350 | (1 of 1) K15287 - solute carrier family 35, member F1/2 (SLC35F1_2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCAGCACGAACCAGCCCTCTGTGCCGCCAAACCCCCCGAAAAACGCGA |
| Internal bar code: | GGGTCAGGCGTTATGTAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 475 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCAAACTGAACCAAACC |
| Suggested primer 2: | CCTGTTGCTTTCGATACGCG |