| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.023347 |
| Chromosome: | chromosome 7 |
| Location: | 112895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312900 | (1 of 1) K10590 - E3 ubiquitin-protein ligase TRIP12 (TRIP12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGCGGCAGATGGACGACGAGGTGGCGCAGCTATCTGCGCTGTCGGAG |
| Internal bar code: | AGTAGCTTGGACTTTCTCGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3329 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTCTTGCCCCCAACTTC |
| Suggested primer 2: | CAGAAGCCTAACCCCAGTGG |