| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.023372 |
| Chromosome: | chromosome 5 |
| Location: | 2764019 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240750 | TEF17 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGAGGCAGAACTGCAAGCTGCCAATAAGGCCTGCTCCCATCTCGACTC |
| Internal bar code: | TGGTTGCGCTAGGGGGTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2381 |
| LEAP-Seq percent confirming: | 94.2857 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGCAGAGGTGTTCACGT |
| Suggested primer 2: | GAAAACGGGCCTGGTTTGTC |