Insertion junction: CLIP2.023387_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023387
Chromosome:chromosome 4
Location:1260381
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g215001 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTACCTCTTTCCACTTATGCTAACAACGTCACGCACACGCACACACAC
Internal bar code:ATCGTCTCGACTGGCTCGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:78
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGCAAAAGAGCGAGGAGGAG
Suggested primer 2:GTTGTTTGCCCACCATTCCC