Insertion junction: CLIP2.023424_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.023424
Chromosome:chromosome 1
Location:4445526
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g030400 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCCAGAGCGGCCAGCGCTGTGCTTGCCGCCTCCTCCAAGGCTCCTCT
Internal bar code:AATTTTAGATTAGTAACCAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2707
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGAGACGATGTCTACACG
Suggested primer 2:TCCTCCTCCAGCTCTACCAC