| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023454 |
| Chromosome: | chromosome 10 |
| Location: | 4910882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g453766 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGAGGCTATAGAGATGCATGTGACGCCGCCGTGTAAACTGACGGTAG |
| Internal bar code: | CATGTTCTCCGCGACGATTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5980 |
| LEAP-Seq percent confirming: | 4.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 150 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTCGCAACTGCTAAGGT |
| Suggested primer 2: | CCCCTCCCCCACATACCTAA |