| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023458 |
| Chromosome: | chromosome 4 |
| Location: | 36133 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213985 | (1 of 62) PF00226 - DnaJ domain (DnaJ) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTGTGGCGGTGCTGGTGCTGGTGCTGGTGCTGGTGCTGGTGCTGGTG |
| Internal bar code: | CGATTTAATTCCGTGGGTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3531 |
| LEAP-Seq percent confirming: | 97.7941 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAACGGAATGTGCACACAC |
| Suggested primer 2: | ACATCGCGCACACTATGACA |